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NCOA1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Nuclear receptor coactivator 1

Gene Aliases

BHLHe42; bHLHe74; class E basic helix-loop-helix protein 74; F-SRC-1; Hin-2 protein; KAT13A; nuclear receptor coactivator 1; Protein Hin-2; renal carcinoma antigen NY-REN-52; RIP160; SRC1; Steroid receptor coactivator 1; steroid receptor coactivator-1.

Gene ID

8648

Accession Number

NP_003734.3

Reactivity

Homo sapiens, Human

Target

Nuclear receptor coactivator 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NCOA1 ELISA Kit (Human) (OKEH04299) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NCOA1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NCOA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NCOA1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

0.056 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

157 kDa

Specificity

Natural and recombinant Human Nuclear receptor coactivator 1

Protein Length

1441

NCBI Gene Symbol

NCOA1

Protein Name

Q15788

Gene Name URL

NCOA1

Nucleotide Accession Number

NM_003743.4

Frequently Asked Questions

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