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NCOR2 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Nuclear receptor co-repressor 2

Gene Aliases

N-CoR; N-CoR2; nuclear receptor corepressor 2; silencing mediator of retinoic acid and thyroid hormone receptor; silencing mediator of retinoid acid and thyroid hormone receptor; SM; SMR; SMRT; SMRTe; T3 receptor-associating factor; thyroid-, retinoic-acid-receptor-associated corepressor; TRAC.

Gene ID

20602

Accession Number

NP_001240833.1

Reactivity

Mouse, Mus musculus

Target

Nuclear receptor corepressor 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Ncor2 ELISA Kit (Mouse) (OKEH04297) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ncor2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ncor2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ncor2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

62.5-4000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%

Sensitivity

31.28 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

270 kDa

Specificity

Natural and recombinant Mouse Nuclear receptor corepressor 2

Protein Length

2472

NCBI Gene Symbol

Ncor2

Protein Name

Q9WU42

Gene Name URL

Ncor2

Nucleotide Accession Number

NM_001253904.1

Frequently Asked Questions

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