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S1PR3 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Sphingosine-1-phosphate receptor 3

Gene Aliases

BA791O21.3; C9orf108; C9orf47; EDG3; EDG-3; endothelial differentiation G-protein coupled receptor 3; endothelial differentiation, sphingolipid G-protein-coupled receptor, 3; G protein-coupled receptor, endothelial differentiation gene-3; LPB3; S1P receptor 3; S1P receptor Edg-3; S1P receptor EDG3; S1P3; sphingosine 1-phosphate receptor 3; sphingosine 1-phosphate receptor Edg-3; uncharacterized protein C9orf47.

Gene ID

1903

Accession Number

NP_005217.2

Reactivity

Homo sapiens, Human

Target

Sphingosine 1-phosphate receptor 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology S1PR3 ELISA Kit (Human) (OKEH04291) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S1PR3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for S1PR3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S1PR3 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

42 kDa

Specificity

Natural and recombinant Human Sphingosine 1-phosphate receptor 3

Protein Length

378

NCBI Gene Symbol

S1PR3

Protein Name

Q99500

Gene Name URL

S1PR3

Nucleotide Accession Number

NM_005226.3

Frequently Asked Questions

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