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CDC42 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Cell division cycle 42

Gene Aliases

CDC42Hs; cell division control protein 42 homolog; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD) ) ; dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD) ) ; G25K; G25K GTP-binding protein; growth-regulating protein; GTP binding protein, 25kDa; small GTP binding protein CDC42; TKS.

Gene ID

998

Accession Number

NP_001034891.1

Reactivity

Homo sapiens, Human

Target

Cell division control protein 42 homolog

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CDC42 ELISA Kit (Human) (OKEH04288) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDC42 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDC42 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDC42 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%

Sensitivity

10 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

21 kDa

Specificity

Natural and recombinant Human Cell division control protein 42 homolog

Protein Length

191

NCBI Gene Symbol

CDC42

Protein Name

P60953

Gene Name URL

CDC42

Nucleotide Accession Number

NM_001039802.1

Frequently Asked Questions

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