IGF2R ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Insulin-like growth factor 2 receptor
Gene Aliases
300 kDa mannose 6-phosphate receptor; AI661837; cation-independent mannose-6-phosphate receptor; cation-independent MPR; CD222; CI-; CI Man-6-P receptor; CI-MPR; IGF-II receptor; IGF-II/CI-MPR; Insulin-like growth factor 2 receptor; insulin-like growth factor II receptor; M6P/IG; M6P/IGF2 receptor; M6P/IGF2R; M6PR; mannose-6-phosphate receptor, cation independent; MPR 300; Mpr3; Mpr300.
Gene ID
16004
Accession Number
NP_034645.2
Reactivity
Mouse, Mus musculus
Target
Cation-independent mannose-6-phosphate receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Igf2r ELISA Kit (Mouse) (OKEH04275) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Igf2r has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Igf2r is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Igf2r captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.087 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
274 kDa
Specificity
Natural and recombinant Mouse Cation-independent mannose-6-phosphate receptor
Protein Length
2483
NCBI Gene Symbol
Igf2r
Protein Name
Q07113
Gene Name URL
Igf2r
Nucleotide Accession Number
NM_010515.2
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
EK6690 48 Tests
Human Serine/threonine-protein kinase 24 (STK24) ELISA Kit
Sign In for Pricing
View Details 
MBS6164065-01 0.1 mL
DCP1A (DCP1 decapping Enzyme Homolog A (S. cerevisiae), FLJ21691, HSA275986, Nbla00360, SMAD4IP1, SMIF) (AP)
Sign In for Pricing
View Details MBS6164065-02 5x 0.1 mL
DCP1A (DCP1 decapping Enzyme Homolog A (S. cerevisiae), FLJ21691, HSA275986, Nbla00360, SMAD4IP1, SMIF) (AP)
Sign In for Pricing
View Details 