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FOLR1 ELISA Kit (Mouse)

Selected Variant

OKEH04258-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Folate receptor 1 (adult)

Gene Aliases

FBP; FBP1; Fol; Folate receptor 1; folate receptor alpha; folate-binding protein 1; Folb; Folbp1; Folbp-1; FR-alpha.

Gene ID

14275

Accession Number

NP_001239481.1

Reactivity

Mouse, Mus musculus

Target

Folate receptor alpha

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Folr1 ELISA Kit (Mouse) (OKEH04258) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Folr1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Folr1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Folr1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

8.1 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

29 kDa

Specificity

Natural and recombinant Mouse Folate receptor alpha

Protein Length

255

NCBI Gene Symbol

Folr1

Protein Name

P35846

Gene Name URL

Folr1

Nucleotide Accession Number

NM_001252552.1

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