FCGR3 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Fc receptor, IgG, low affinity III
Gene Aliases
Fc-gamma RIII.
Gene ID
14131
Accession Number
NP_034318.2
Reactivity
Mouse, Mus musculus
Target
Low affinity immunoglobulin gamma Fc region receptor III
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Fcgr3 ELISA Kit (Mouse) (OKEH04249) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fcgr3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fcgr3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fcgr3 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.19 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
30 kDa
Specificity
Natural and recombinant Mouse Low affinity immunoglobulin gamma Fc region receptor III
Protein Length
261
NCBI Gene Symbol
FCGR3
Protein Name
P08508
Gene Name URL
FCGR3
Nucleotide Accession Number
NM_010188.5
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
bsm-52187R-PE 100 µL
Phospho-PKC alpha (Thr638) Recombinant Antibody, PE Conjugated
Sign In for Pricing
View Details 
MBS6310876-01 0.2 mL
IREB2, ID (IREB2, Iron-responsive element-binding protein 2, Iron regulatory protein 2) (Azide free) (HRP)
Sign In for Pricing
View Details MBS6310876-02 5x 0.2 mL
IREB2, ID (IREB2, Iron-responsive element-binding protein 2, Iron regulatory protein 2) (Azide free) (HRP)
Sign In for Pricing
View Details 
orb1579311 100 µL
Phospho-eNOS (Thr495) Rabbit Polyclonal Antibody (BF680)
Sign In for Pricing
View Details