FCGR2 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Fc receptor, IgG, low affinity IIb
Gene Aliases
AI528646; CD32; F630109E10Rik; fc gamma receptor IIB; Fc gamma RIIB; Fc[g]; Fc[g]RII; Fcg; Fcgamm; fc-gamma RII; Fc-gamma-RII; fc-gamma-RIIB; FcgammaRIIB; Fcgr; Fcgr2; Fcgr2a; FcgRII; Fcr-; Fcr-2; Fcr-3; fcRII; igG Fc receptor II beta; low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; Ly-1; Ly-17; LyM-; LyM-1; Ly-m2; Ly-m20; lymphocyte antigen 17.
Gene ID
14130
Accession Number
NP_034317.1
Reactivity
Mouse, Mus musculus
Target
Low affinity immunoglobulin gamma Fc region receptor II
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Fcgr2 ELISA Kit (Mouse) (OKEH04248) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fcgr2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fcgr2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fcgr2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.188 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
37 kDa
Specificity
Natural and recombinant Mouse Low affinity immunoglobulin gamma Fc region receptor II
Protein Length
330
NCBI Gene Symbol
Fcgr2b
Protein Name
P08101
Gene Name URL
Fcgr2b
Nucleotide Accession Number
NM_010187.2
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