What is the catalog number for H2-T3 ELISA Kit (Mouse)?

The catalog number for H2-T3 ELISA Kit (Mouse) is OKEH04239-96W.

What sizes are available for H2-T3 ELISA Kit (Mouse)?

H2-T3 ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is H2-T3 ELISA Kit (Mouse) in stock?

H2-T3 ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

Is there an additional fee for hazardous or dry ice shipping of this product?

Yes, if this product requires dry ice or is classed as hazardous for transport, the additional fee will be explicitly shown in your quotation before you confirm the order, so there are no surprise charges later.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

H2-T3 ELISA Kit (Mouse)

Selected Variant

OKEH04239-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Histocompatibility 2, T region locus 3

Gene Aliases

MHC thymus leukemia antigen.

Gene ID

15043

Accession Number

NP_032234.3

Reactivity

Mouse, Mus musculus

Target

H-2 class I histocompatibility antigen, TLA (B) alpha chain

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology H2-T3 ELISA Kit (Mouse) (OKEH04239) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for H2-T3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for H2-T3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample H2-T3 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.312-20ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%

Sensitivity

0.176 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

43 kDa

Specificity

Natural and recombinant Mouse H-2 class I histocompatibility antigen, TLA (B) alpha chain

Protein Length

384

NCBI Gene Symbol

H2-T3

Protein Name

P14432

Gene Name URL

H2-T3

Nucleotide Accession Number

NM_008208.4

Price

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