MACC1 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
MET transcriptional regulator MACC1
Gene Aliases
7A5; MACC1, MET transcriptional regulator; metastasis associated in colon cancer 1; metastasis-associated in colon cancer protein 1; putative binding protein 7a5; SH3 domain-containing protein 7a5; SH3BP4L.
Gene ID
346389
Accession Number
NP_877439.3
Reactivity
Homo sapiens, Human
Target
Metastasis-associated in colon cancer protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MACC1 ELISA Kit (Human) (OKEH04233) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MACC1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MACC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MACC1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
Sensitivity
0.054 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
97 kDa
Specificity
Natural and recombinant Human Metastasis-associated in colon cancer protein 1
Protein Length
852
NCBI Gene Symbol
MACC1
Protein Name
Q6ZN28
Gene Name URL
MACC1
Nucleotide Accession Number
NM_182762.3
Frequently Asked Questions
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