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MACC1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

MET transcriptional regulator MACC1

Gene Aliases

7A5; MACC1, MET transcriptional regulator; metastasis associated in colon cancer 1; metastasis-associated in colon cancer protein 1; putative binding protein 7a5; SH3 domain-containing protein 7a5; SH3BP4L.

Gene ID

346389

Accession Number

NP_877439.3

Reactivity

Homo sapiens, Human

Target

Metastasis-associated in colon cancer protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MACC1 ELISA Kit (Human) (OKEH04233) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MACC1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MACC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MACC1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%

Sensitivity

0.054 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

97 kDa

Specificity

Natural and recombinant Human Metastasis-associated in colon cancer protein 1

Protein Length

852

NCBI Gene Symbol

MACC1

Protein Name

Q6ZN28

Gene Name URL

MACC1

Nucleotide Accession Number

NM_182762.3

Frequently Asked Questions

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