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ZFAND6 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Zinc finger AN1-type containing 6

Gene Aliases

AN1-type zinc finger protein 6; Associated with PRK1 protein; AWP1; protein associated with PRK1; ZA20D3; ZFAND5B; Zinc finger A20 domain-containing protein 3; zinc finger, A20 domain containing 3; zinc finger, AN1-type domain 6.

Gene ID

54469

Accession Number

NP_001229840.1

Reactivity

Homo sapiens, Human

Target

AN1-type zinc finger protein 6

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ZFAND6 ELISA Kit (Human) (OKEH04232) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ZFAND6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ZFAND6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ZFAND6 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

1.56-100U/L

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%

Sensitivity

0.78U/L

Reconstitution

Store as indicated in product manual.

Molecular Weight

23 kDa

Specificity

Natural and recombinant Human AN1-type zinc finger protein 6

Protein Length

208

NCBI Gene Symbol

ZFAND6

Protein Name

Q6FIF0

Gene Name URL

ZFAND6

Nucleotide Accession Number

NM_001242911.1

Frequently Asked Questions

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