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ZMAT3 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Zinc finger matrin-type 3

Gene Aliases

P53 target zinc finger protein; p53-activated gene 608 protein; PAG608; WIG1; WIG-1; WIG-1/PAG608 protein; zinc finger matrin-type protein 3; zinc finger protein WIG1; Zinc finger protein WIG-1.

Gene ID

64393

Accession Number

NP_071915.1

Reactivity

Homo sapiens, Human

Target

Zinc finger matrin-type protein 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ZMAT3 ELISA Kit (Human) (OKEH04229) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ZMAT3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ZMAT3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ZMAT3 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.156-10ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 83%

Sensitivity

0.1 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

32 kDa

Specificity

Natural and recombinant Human Zinc finger matrin-type protein 3

Protein Length

289

NCBI Gene Symbol

ZMAT3

Protein Name

Q9HA38

Gene Name URL

ZMAT3

Nucleotide Accession Number

NM_022470.3

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