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NLRC4 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

NLR family, CARD domain containing 4

Gene Aliases

9530011P19Rik; Card; Card12; caspase recruitment domain family, member 12; caspase recruitment domain-containing protein 12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; I; ice protease-activating factor; IPAF; NLR family CARD domain-containing protein 4.

Gene ID

268973

Accession Number

NP_001028539.1

Reactivity

Mouse, Mus musculus

Target

NLR family CARD domain-containing protein 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Nlrc4 ELISA Kit (Mouse) (OKEH04227) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nlrc4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nlrc4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nlrc4 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.156-10ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%

Sensitivity

0.083 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

117 kDa

Specificity

Natural and recombinant Mouse NLR family CARD domain-containing protein 4

Protein Length

1024

NCBI Gene Symbol

Nlrc4

Protein Name

Q3UP24

Gene Name URL

Nlrc4

Nucleotide Accession Number

NM_001033367.3

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