POU5F1 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
POU domain, class 5, transcription factor 1
Gene Aliases
NF-A3; Oct; Oct-; Oct3; Oct-3; Oct3/; Oct-3/; Oct3/4; Oct-3/4; Oct4; Oct-4; octamer-binding protein 3; octamer-binding protein 4; octamer-binding transcription factor 3; octamer-binding transcription factor 4 alternative; Otf; Otf-; Otf3; Otf3-; Otf-3; Otf3g; Otf3-rs7; Otf4; Otf-4; POU domain class 5 transcription factor 1 transcript variant 2 oct4b'; POU domain class 5 transcription factor 1 transcript variant 3 oct4b; POU domain, class 5, transcription factor 1.
Gene ID
18999
Accession Number
NP_038661.2
Reactivity
Mouse, Mus musculus
Target
POU domain, class 5, transcription factor 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Pou5f1 ELISA Kit (Mouse) (OKEH04225) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pou5f1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pou5f1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pou5f1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
Sensitivity
0.082 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
38 kDa
Specificity
Natural and recombinant Mouse POU domain, class 5, transcription factor 1
Protein Length
352
NCBI Gene Symbol
Pou5f1
Protein Name
P20263
Gene Name URL
Pou5f1
Nucleotide Accession Number
NM_013633.3
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