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SCARB1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Scavenger receptor class B member 1

Gene Aliases

CD36 and LIMPII analogous 1; CD36 antigen (collagen type I receptor, thrombospondin receptor) -like 1; CD36 antigen-like 1; CD36L1; CLA1; CLA-1; Collagen type I receptor, thrombospondin receptor-like 1; HDLQTL6; scavenger receptor class B member 1; scavenger receptor class B type III; SRB1; SR-BI.

Gene ID

949

Accession Number

NP_001076428.1

Reactivity

Homo sapiens, Human

Target

Scavenger receptor class B member 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SCARB1 ELISA Kit (Human) (OKEH04223) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SCARB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SCARB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SCARB1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%

Sensitivity

0.1 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

61 kDa

Specificity

Natural and recombinant Human Scavenger receptor class B member 1

Protein Length

552

NCBI Gene Symbol

SCARB1

Protein Name

Q8WTV0

Gene Name URL

SCARB1

Nucleotide Accession Number

NM_001082959.1

Frequently Asked Questions

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