SCARB1 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Scavenger receptor class B member 1
Gene Aliases
CD36 and LIMPII analogous 1; CD36 antigen (collagen type I receptor, thrombospondin receptor) -like 1; CD36 antigen-like 1; CD36L1; CLA1; CLA-1; Collagen type I receptor, thrombospondin receptor-like 1; HDLQTL6; scavenger receptor class B member 1; scavenger receptor class B type III; SRB1; SR-BI.
Gene ID
949
Accession Number
NP_001076428.1
Reactivity
Homo sapiens, Human
Target
Scavenger receptor class B member 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SCARB1 ELISA Kit (Human) (OKEH04223) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SCARB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SCARB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SCARB1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
Sensitivity
0.1 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
61 kDa
Specificity
Natural and recombinant Human Scavenger receptor class B member 1
Protein Length
552
NCBI Gene Symbol
SCARB1
Protein Name
Q8WTV0
Gene Name URL
SCARB1
Nucleotide Accession Number
NM_001082959.1
Frequently Asked Questions
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