NAIP1 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
NLR family, apoptosis inhibitory protein 1
Gene Aliases
AV364616; baculoviral IAP repeat-containing 1a; baculoviral IAP repeat-containing protein 1a; Bir; Birc1a; D13Ls; D13Lsd1; Naip; Naip-rs1; neuronal apoptosis inhibitory protein 1.
Gene ID
17940
Accession Number
NP_032696.2
Reactivity
Mouse, Mus musculus
Target
Baculoviral IAP repeat-containing protein 1a
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Naip1 ELISA Kit (Mouse) (OKEH04218) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Naip1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Naip1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Naip1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
159 kDa
Specificity
Natural and recombinant Mouse Baculoviral IAP repeat-containing protein 1a
Protein Length
1403
NCBI Gene Symbol
Naip1
Protein Name
Q9QWK5
Gene Name URL
Naip1
Nucleotide Accession Number
NM_008670.2
Frequently Asked Questions
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