HPGDS ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Hematopoietic prostaglandin D synthase
Gene Aliases
Glutathione S-transferase; glutathione-dependent PGD synthase; glutathione-dependent PGD synthetase; glutathione-requiring prostaglandin D synthase; GST class-sigma; hematopoietic prostaglandin D synthase; H-PGDS; prostaglandin D2 synthase 2, hematopoietic; prostaglandin-H2 D-isomerase; Ptgds2.
Gene ID
58962
Accession Number
NP_113832.1
Reactivity
Rat, Rattus norvegicus
Target
Hematopoietic prostaglandin D synthase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Hpgds ELISA Kit (Rat) (OKEH04202) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hpgds has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hpgds is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hpgds captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/ml
Sensitivity
0.092 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
23 kDa
Specificity
Natural and recombinant Rat Hematopoietic prostaglandin D synthase
Protein Length
199
NCBI Gene Symbol
Hpgds
Protein Name
O35543
Gene Name URL
Hpgds
Nucleotide Accession Number
NM_031644.2
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