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HPGDS ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Hematopoietic prostaglandin D synthase

Gene Aliases

Glutathione S-transferase; glutathione-dependent PGD synthase; glutathione-dependent PGD synthetase; glutathione-requiring prostaglandin D synthase; GST class-sigma; hematopoietic prostaglandin D synthase; H-PG; H-PGDS; prostaglandin D2 synthase 2, hematopoietic; prostaglandin-H2 D-isomerase; Ptgd; Ptgds2.

Gene ID

54486

Accession Number

NP_062328.3

Reactivity

Mouse, Mus musculus

Target

Hematopoietic prostaglandin D synthase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology Hpgds ELISA Kit (Mouse) (OKEH04201) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse Hpgds antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Hpgds and incubated. The Hpgds found in the sample or standards competes with the biotinylated Hpgds for limited binding sites on the immobilized anti-mouse Hpgds antibody. Excess unbound biotinylated Hpgds and sample or standard Hpgds is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Hpgds captured in the well and inversely proportional to the amount of Hpgds which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.625-40ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

0.324 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

23 kDa

Specificity

Natural and recombinant Mouse Hematopoietic prostaglandin D synthase

Protein Length

199

NCBI Gene Symbol

Hpgds

Protein Name

Q9JHF7

Gene Name URL

Hpgds

Nucleotide Accession Number

NM_019455.4

Frequently Asked Questions

More Discoveries

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