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DDR2 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Discoidin domain receptor tyrosine kinase 2

Gene Aliases

CD167 antigen-like family member B; cell migration-inducing protein 20; discoidin domain receptor 2; discoidin domain receptor family, member 2; discoidin domain-containing receptor 2; discoidin domain-containing receptor tyrosine kinase 2; hydroxyaryl-protein kinase; MIG20a; migration-inducing gene 16 protein; neurotrophic tyrosine kinase receptor related 3; Neurotrophic tyrosine kinase, receptor-related 3; NTRKR3; receptor protein-tyrosine kinase TKT; TKT; TYRO10; tyrosine-protein kinase TYRO10; WRCN.

Gene ID

4921

Accession Number

NP_001014796.1

Reactivity

Homo sapiens, Human

Target

Discoidin domain-containing receptor 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology DDR2 ELISA Kit (Human) (OKEH04200) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DDR2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DDR2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DDR2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.156-10ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

97 kDa

Specificity

Natural and recombinant Human Discoidin domain-containing receptor 2

Protein Length

855

NCBI Gene Symbol

DDR2

Protein Name

Q16832

Gene Name URL

DDR2

Nucleotide Accession Number

NM_001014796.1

Frequently Asked Questions

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