SLC50A1 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Solute carrier family 50 (sugar transporter), member 1
Gene Aliases
MmSWEET1; probable sugar transporter RAG1AP1; R; Rag; RAG1-activating protein 1; Rag1ap1; recombination activating gene 1 activating protein 1; recombination activating gene 1 gene activation; Rga; solute carrier family 50 member 1; sugar transporter SWEET1.
Gene ID
19729
Accession Number
NP_033083.2
Reactivity
Mouse, Mus musculus
Target
Sugar transporter SWEET1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Slc50a1 ELISA Kit (Mouse) (OKEH04197) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc50a1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Slc50a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc50a1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
Sensitivity
39.4 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
25 kDa
Specificity
Natural and recombinant Mouse Sugar transporter SWEET1
Protein Length
221
NCBI Gene Symbol
Slc50a1
Protein Name
Q9CXK4
Gene Name URL
Slc50a1
Nucleotide Accession Number
NM_009057.3
Frequently Asked Questions
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