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ESRRG ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Estrogen-related receptor gamma

Gene Aliases

Errg; estrogen receptor-related protein 3; estrogen-related receptor gamma; nuclear receptor subfamily 3 group B member 3.

Gene ID

360896

Accession Number

NP_976081.1

Reactivity

Rat, Rattus norvegicus

Target

Estrogen-related receptor gamma

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Esrrg ELISA Kit (Rat) (OKEH04186) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Esrrg has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Esrrg is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Esrrg captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.312-20ng/mL

Sensitivity

0.177 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

51 kDa

Specificity

Natural and recombinant Rat Estrogen-related receptor gamma

Protein Length

458

NCBI Gene Symbol

Esrrg

Protein Name

P62510

Gene Name URL

Esrrg

Nucleotide Accession Number

NM_203336.2

Frequently Asked Questions

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