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CCBL1 ELISA Kit (Rat)

Selected Variant

OKEH04184-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Kynurenine aminotransferase 1

Gene Aliases

Ccbl1; cysteine conjugate-beta lyase 1; cysteine conjugate-beta lyase, cytoplasmic; cysteine-S-conjugate beta-lyase; glutamine transaminase K; glutamine--phenylpyruvate transaminase; Gtk; Kat1; KatI; Kynurenine aminotransferase 1; kynurenine aminotransferase I; kynurenine aminotransferase/glutamine transaminase K; kynurenine--oxoglutarate transaminase 1; kynurenine--oxoglutarate transaminase 1, mitochondrial; kynurenine--oxoglutarate transaminase I.

Gene ID

311844

Accession Number

NP_001013182.3

Reactivity

Rat, Rattus norvegicus

Target

Kynurenine--oxoglutarate transaminase 1, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Ccbl1 ELISA Kit (Rat) (OKEH04184) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccbl1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccbl1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ccbl1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.156-10ng/ml

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

52 kDa

Specificity

Natural and recombinant Rat Kynurenine--oxoglutarate transaminase 1, mitochondrial

Protein Length

457

NCBI Gene Symbol

Kyat1

Protein Name

Q08415

Gene Name URL

Kyat1

Nucleotide Accession Number

NM_001013164.3

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