ITGAV ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Integrin subunit alpha V
Gene Aliases
Antigen identified by monoclonal antibody L230; CD51; integrin alpha-V; integrin alphaVbeta3; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) ; MSK8; Vitronectin receptor; vitronectin receptor subunit alpha; VNRA; VTNR.
Gene ID
3685
Accession Number
NP_001138471.1
Reactivity
Homo sapiens, Human
Target
Integrin alpha-V
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ITGAV ELISA Kit (Human) (OKEH04178) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ITGAV has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ITGAV is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ITGAV captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
Sensitivity
0.11 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
116 kDa
Specificity
Natural and recombinant Human Integrin alpha-V
Protein Length
1048
NCBI Gene Symbol
ITGAV
Protein Name
P06756
Gene Name URL
ITGAV
Nucleotide Accession Number
NM_001144999.2
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