MAT1A ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Methionine adenosyltransferase 1A
Gene Aliases
AdoMet; adoMet synthase 1; adoMet synthetase 1; MAT 1; MAT-I/III; methionine adenosyltransferase 1; methionine adenosyltransferase I, alpha; methionine adenosyltransferase I/III; S - adenosylmethionine synthetase; SADE; S-adenosylmethionine synthase isoform type-1; SAS.
Gene ID
25331
Accession Number
NP_036992.2
Reactivity
Rat, Rattus norvegicus
Target
S-adenosylmethionine synthase isoform type-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Mat1a ELISA Kit (Rat) (OKEH04170) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mat1a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mat1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mat1a captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
0.163 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
44 kDa
Specificity
Natural and recombinant Rat S-adenosylmethionine synthase isoform type-1
Protein Length
397
NCBI Gene Symbol
Mat1a
Protein Name
P13444
Gene Name URL
Mat1a
Nucleotide Accession Number
NM_012860.2
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