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IL17RB ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Interleukin 17 receptor B

Gene Aliases

CRL4; cytokine receptor CRL4; cytokine receptor-like 4; EVI27; IL-17 receptor B; IL-17 receptor homolog 1; IL-17B receptor; IL17BR; IL-17RB; IL-17Rh1; IL17RH1; interleukin 17 receptor homolog 1; interleukin-17 receptor B; interleukin-17B receptor.

Gene ID

55540

Accession Number

NP_061195.2

Reactivity

Homo sapiens, Human

Target

Interleukin-17 receptor B

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology IL17RB ELISA Kit (Human) (OKEH04169) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL17RB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL17RB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL17RB captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

0.065 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

56 kDa

Specificity

Natural and recombinant Human Interleukin-17 receptor B

Protein Length

502

NCBI Gene Symbol

IL17RB

Protein Name

Q9NRM6

Gene Name URL

IL17RB

Nucleotide Accession Number

NM_018725.3

Frequently Asked Questions

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