MYH9 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Myosin heavy chain 9
Gene Aliases
BDPLT6; cellular myosin heavy chain, type A; DFNA17; EPSTS; FTNS; MATINS; MHA; Myosin heavy chain 9; Myosin heavy chain, non-muscle IIa; myosin, heavy chain 9, non-muscle; myosin-9; NMHC-II-A; NMMHCA; NMMHC-IIA; non-muscle myosin heavy chain 9; non-muscle myosin heavy chain A; non-muscle myosin heavy chain IIa; nonmuscle myosin heavy chain II-A; non-muscle myosin heavy polypeptide 9.
Gene ID
4627
Accession Number
NP_002464.1
Reactivity
Homo sapiens, Human
Target
Myosin-9
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MYH9 ELISA Kit (Human) (OKEH04168) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MYH9 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MYH9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MYH9 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
15.6-1000pg/mL
Recovery
<= 4.9%
Sensitivity
10 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
227 kDa
Specificity
Natural and recombinant Human Myosin-9
Protein Length
1960
NCBI Gene Symbol
MYH9
Protein Name
P35579
Gene Name URL
MYH9
Nucleotide Accession Number
NM_002473.5
Frequently Asked Questions
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