KLF13 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Kruppel like factor 13
Gene Aliases
Basic transcription element binding protein 3; Basic transcription element-binding protein 3; BTEB3; BTE-binding protein 3; FKLF2; Krueppel-like factor 13; novel Sp1-like zinc finger transcription factor 1; NSLP1; RANTES factor of late activated T lymphocytes-1; RANTES factor of late activated T-lymphocytes 1; RFLAT1; RFLAT-1; transcription factor BTEB3; transcription factor NSLP1.
Gene ID
51621
Accession Number
NP_001289390.1
Reactivity
Homo sapiens, Human
Target
Krueppel-like factor 13
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology KLF13 ELISA Kit (Human) (OKEH04165) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KLF13 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KLF13 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KLF13 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
Sensitivity
0.087 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
31 kDa
Specificity
Natural and recombinant Human Krueppel-like factor 13
Protein Length
288
NCBI Gene Symbol
KLF13
Protein Name
Q9Y2Y9
Gene Name URL
KLF13
Nucleotide Accession Number
NM_001302461.1
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