TPSD1 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
Gene Name
Tryptase delta 1
Gene Aliases
Delta-tryptase; hmMCP-3-like tryptase III; Mast cell mMCP-7-like; mast cell tryptase; MCP7L1; MCP7-LIKE; MMCP-7L; mMCP-7-like delta II tryptase; mMCP-7-like-1; mMCP-7-like-2; tryptase delta; tryptase-3.
Gene ID
23430
Accession Number
NP_036349.1
Reactivity
Homo sapiens, Human
Target
Tryptase delta
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TPSD1 ELISA Kit (Human) (OKEH04155) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TPSD1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TPSD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TPSD1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Sensitivity
32 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
27 kDa
Specificity
Natural and recombinant Human Tryptase delta
Protein Length
242
NCBI Gene Symbol
TPSD1
Protein Name
Q9BZJ3
Gene Name URL
TPSD1
CAS Number
7732-18-5
Nucleotide Accession Number
NM_012217.2
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