NTF3 ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
Gene Name
Neurotrophin 3
Gene Aliases
HDNF; nerve growth factor 2; neurotrophic factor; neurotrophin-3; neurotrophin-3 (HDNF/NT-3) ; NGF-2; NT-3.
Gene ID
81737
Accession Number
NP_001257797.1
Reactivity
Rat, Rattus norvegicus
Target
Neurotrophin-3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ntf3 ELISA Kit (Rat) (OKEH04139) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ntf3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ntf3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ntf3 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
30 kDa
Specificity
Natural and recombinant Rat Neurotrophin-3
Protein Length
258
NCBI Gene Symbol
Ntf3
Protein Name
P18280
Gene Name URL
Ntf3
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001270868.1
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
MBS6190130-01 0.1 mL
FBXO7 (F-box Only Protein 7, FBX7, FBX, FBX07, DKFZp686B08113, PARK15, PKPS) (MaxLight 405)
Ask
View Details MBS6190130-02 5x 0.1 mL
FBXO7 (F-box Only Protein 7, FBX7, FBX, FBX07, DKFZp686B08113, PARK15, PKPS) (MaxLight 405)
Ask
View Details