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ERBB2 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Erb-b2 receptor tyrosine kinase 2

Gene Aliases

Avian erythroblastosis oncogene B 2; c-erbB; c-erbB2; c-erbB-2; c-ne; c-neu; ErbB-; Erbb-2; HER; HER-; HER2; HER-2; l11Ju; l11Jus8; mKIAA3023; Ne; Neu; Neu oncogene; p185erbB2; proto-oncogene c-ErbB-2; proto-oncogene NEU; receptor tyrosine-protein kinase erbB-2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog.

Gene ID

13866

Accession Number

NP_001003817.1

Reactivity

Mouse, Mus musculus

Target

Receptor tyrosine-protein kinase erbB-2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Erbb2 ELISA Kit (Mouse) (OKEH04115) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Erbb2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Erbb2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Erbb2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%

Sensitivity

0.079 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

139 kDa

Specificity

Natural and recombinant Mouse Receptor tyrosine-protein kinase erbB-2

Protein Length

1256

NCBI Gene Symbol

Erbb2

Protein Name

P70424

Gene Name URL

Erbb2

Nucleotide Accession Number

NM_001003817.1

Frequently Asked Questions

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