RETN ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Resistin
Gene Aliases
AD; adipose tissue-specific secretory factor; adipose-specific cysteine-rich secreted protein A12-alpha; ADSF; cysteine-rich secreted protein FIZZ3; dominant inhibitory adipocyte-specific secretory factor; Fizz; Fizz3; found in inflammatory zone 3; r; resistin; Rstn; Xcp; Xcp4.
Gene ID
57264
Accession Number
NP_001191888.1
Reactivity
Mouse, Mus musculus
Target
Resistin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Retn ELISA Kit (Mouse) (OKEH04076) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Retn has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Retn is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Retn captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%
Sensitivity
0.088 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
12 kDa
Specificity
Natural and recombinant Mouse Resistin
Protein Length
114
NCBI Gene Symbol
Retn
Protein Name
Q99P87
Gene Name URL
Retn
Nucleotide Accession Number
NM_001204959.1
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