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PF4 ELISA Kit (Mouse)

Selected Variant

OKEH04061-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Platelet factor 4

Gene Aliases

Chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; Cxcl; Cxcl4; Pf; PF-4; platelet factor 4; Scyb; Scyb4.

Gene ID

56744

Accession Number

NP_064316.1

Reactivity

Mouse, Mus musculus

Target

Platelet factor 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Pf4 ELISA Kit (Mouse) (OKEH04061) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pf4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pf4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pf4 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%

Sensitivity

39.8 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

11 kDa

Specificity

Natural and recombinant Mouse Platelet factor 4

Protein Length

105

NCBI Gene Symbol

Pf4

Protein Name

Q9Z126

Gene Name URL

Pf4

Nucleotide Accession Number

NM_019932.4

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