TNFRSF1B ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Tumor necrosis factor receptor superfamily, member 1b
Gene Aliases
CD120b; p7; p75; p75 TNFR; p80 TNF-alpha receptor; TN; Tnf; TNF-; TNF receptor beta chain; TNF-a; TNFal; TNFalpha-R2; TNF-alphaR2; TNFBR; TNFR; TNF-R; Tnfr-1; Tnfr2; TNF-R2; TNF-R75; TNFR80; TNFRII; TNFR-II; TNF-RII; TNF-R-II; tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor type II.
Gene ID
21938
Accession Number
NP_035740.2
Reactivity
Mouse, Mus musculus
Target
Tumor necrosis factor receptor superfamily member 1B
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Tnfrsf1b ELISA Kit (Mouse) (OKEH04057) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tnfrsf1b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tnfrsf1b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tnfrsf1b captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 83%
Sensitivity
7.83 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
50 kDa
Specificity
Natural and recombinant Mouse Tumor necrosis factor receptor superfamily member 1B
Protein Length
474
NCBI Gene Symbol
Tnfrsf1b
Protein Name
P25119
Gene Name URL
Tnfrsf1b
Nucleotide Accession Number
NM_011610.3
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
125577 100 µg
CYP2C8 (Cytochrome P450 2C8, CYPIIC8, Cytochrome P450 IIC2, Cytochrome P450 MP-12, Cytochrome P450 MP-20, Cytochrome P450 form 1, S-mephenytoin 4-hydroxylase)
Ask
View Details 
40577121 3x 1.0µg DNA
RNU7-64P CRISPRa sgRNA lentivector (set of three targets)(Human)
Ask
View Details