TNFRSF1B ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Tumor necrosis factor receptor superfamily, member 1b

Gene Aliases

CD120b; p7; p75; p75 TNFR; p80 TNF-alpha receptor; TN; Tnf; TNF-; TNF receptor beta chain; TNF-a; TNFal; TNFalpha-R2; TNF-alphaR2; TNFBR; TNFR; TNF-R; Tnfr-1; Tnfr2; TNF-R2; TNF-R75; TNFR80; TNFRII; TNFR-II; TNF-RII; TNF-R-II; tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor type II.

Gene ID

21938

Accession Number

NP_035740.2

Reactivity

Mouse, Mus musculus

Target

Tumor necrosis factor receptor superfamily member 1B

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Tnfrsf1b ELISA Kit (Mouse) (OKEH04057) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tnfrsf1b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tnfrsf1b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tnfrsf1b captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol