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TNFRSF1A ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Tumor necrosis factor receptor superfamily, member 1a

Gene Aliases

CD120; CD120a; FPF; p5; p55; p55-R; p60; TN; Tnf; TNF-; TNF receptor alpha chain; TNF-a; TNFal; TNFalpha-R1; TNF-alphaR1; TNF-alpha-R1; TNFAR; TNFR; TNF-R; Tnfr1; TNF-R1; Tnfr-2; TNF-R55; TNFR60; TNFRI; TNFR-I; TNF-RI; TNF-R-I; TNFRp55; tumor necrosis factor receptor 1; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor type I.

Gene ID

21937

Accession Number

NP_035739.2

Reactivity

Mouse, Mus musculus

Target

Tumor necrosis factor receptor superfamily member 1A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Tnfrsf1a ELISA Kit (Mouse) (OKEH04055) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tnfrsf1a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tnfrsf1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tnfrsf1a captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.078-5ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 83%

Sensitivity

0.039 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

50 kDa

Specificity

Natural and recombinant Mouse Tumor necrosis factor receptor superfamily member 1A

Protein Length

454

NCBI Gene Symbol

Tnfrsf1a

Protein Name

P25118

Gene Name URL

Tnfrsf1a

Nucleotide Accession Number

NM_011609.4

Frequently Asked Questions

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