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CCL19 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Chemokine (C-C motif) ligand 19, predicted gene 2023

Gene Aliases

C-C motif chemokine 19; Ccl19; chemokine CCL19; CKb11; EBI1 ligand chemokine; EBI-1 ligand chemokine; EBV-induced molecule 1 ligand chemokine; ELC; epstein-Barr virus-induced molecule 1 ligand chemokine; exodus; exodus-3; Gm2023; MIP3B; Scya; Scya19; small-inducible cytokine A19.

Gene ID

100039053, 24047

Accession Number

NP_036018.1

Reactivity

Mouse, Mus musculus

Target

C-C motif chemokine 19

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Ccl19 ELISA Kit (Mouse) (OKEH04049) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccl19 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccl19 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ccl19 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%

Sensitivity

7.93 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

12 kDa

Specificity

Natural and recombinant Mouse C-C motif chemokine 19

Protein Length

108

NCBI Gene Symbol

Ccl19, Gm2023

Protein Name

O70460

Gene Name URL

Ccl19, Gm2023

Nucleotide Accession Number

NM_011888.2

Frequently Asked Questions

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