TFRC ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
Gene Name
Transferrin receptor
Gene Aliases
CD71; IMD46; p90; T9; TFR; TFR1; TR; transferrin receptor protein 1; TRFR.
Gene ID
7037
Accession Number
NP_001121620.1
Reactivity
Homo sapiens, Human
Target
Transferrin receptor protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TFRC ELISA Kit (Human) (OKEH04014) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TFRC has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TFRC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TFRC captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
3.12-200ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
1 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
85 kDa
Specificity
Natural and recombinant Human Transferrin receptor protein 1
Protein Length
760
NCBI Gene Symbol
TFRC
Protein Name
P02786
Gene Name URL
TFRC
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001128148.2
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
GTR15174528 1 Vial
Lentiviral mouse Tiam2 shRNA (UAS) - Lentiviral mouse Tiam2 shRNA (UAS, RFP) (100)
Ask
View Details 
PC1008061 250 mg
(4- (5- (Trifluoromethyl) -1,2,4-oxadiazol-3-yl) phenyl) methanamine hydrochloride
Ask
View Details ![EIF4E2 Mouse Monoclonal Antibody [KD Validation]](https://cdn.genprice.uk/genprice/genprice4.png)
