TERT ELISA Kit (Dog)
Product Specifications
CAS Number
7732-18-5
Gene Name
Telomerase reverse transcriptase
Gene Aliases
Telomerase catalytic subunit; telomerase reverse transcriptase; telomerase reverse transcriptase subunit.
Gene ID
403412
Accession Number
NP_001026800.1
Reactivity
Canis lupus, Dog
Target
Telomerase reverse transcriptase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TERT ELISA Kit (Dog) (OKEH03979) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TERT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TERT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TERT captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Sensitivity
17 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
1123 kDa
Specificity
Natural and recombinant Dog Telomerase reverse transcriptase
Protein Length
1123
NCBI Gene Symbol
TERT
Protein Name
Q6A548
Gene Name URL
TERT
Nucleotide Accession Number
NM_001031630.1
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