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NOS3 ELISA Kit (Dog)

Product Specifications

CAS Number

7732-18-5

Gene Name

Nitric oxide synthase 3 (endothelial cell)

Gene Aliases

Constitutive NOS; EC-NOS; Endothelial NOS; NOS type III.

Gene ID

403784

Accession Number

NP_001003158.2

Reactivity

Canis lupus, Dog

Target

Nitric oxide synthase, endothelial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NOS3 ELISA Kit (Dog) (OKEH03970) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NOS3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NOS3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NOS3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

3.12-200U/mL

Sensitivity

1.37U/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

1205 kDa

Specificity

Natural and recombinant Dog Nitric oxide synthase, endothelial

Protein Length

1205

NCBI Gene Symbol

NOS3

Protein Name

Q9TUX8

Gene Name URL

NOS3

Nucleotide Accession Number

NM_001003158.2

Frequently Asked Questions

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