EGF ELISA Kit (Dog)
Product Specifications
CAS Number
7732-18-5
Gene Name
Epidermal growth factor
Gene Aliases
CEGF; epidermal growth factor (beta-urogastrone) ; pro-epidermal growth factor.
Gene ID
403657
Accession Number
NP_001003094.1
Reactivity
Canis lupus, Dog
Target
Pro-epidermal growth factor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology EGF ELISA Kit (Dog) (OKEH03965) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EGF has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EGF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EGF captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/mL
Sensitivity
10.6 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
1216 kDa
Specificity
Natural and recombinant Dog Pro-epidermal growth factor
Protein Length
1216
NCBI Gene Symbol
EGF
Protein Name
Q9BEA0
Gene Name URL
EGF
Nucleotide Accession Number
NM_001003094.1
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