GPLD1 ELISA Kit (Bovine)

Product Specifications

CAS Number

7732-18-5

Gene Name

Glycosylphosphatidylinositol specific phospholipase D1

Gene Aliases

Glycoprotein phospholipase D; glycosyl-phosphatidylinositol-specific phospholipase D; GPI-PLD; GPI-specific phospholipase D; phosphatidylinositol-glycan-specific phospholipase D; PI-G PLD.

Gene ID

287025

Accession Number

NP_777241.1

Reactivity

Bos taurus, Bovine

Target

Phosphatidylinositol-glycan-specific phospholipase D

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology GPLD1 ELISA Kit (Bovine) (OKEH03927) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human GPLD1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GPLD1 and incubated. The GPLD1 found in the sample or standards competes with the biotinylated GPLD1 for limited binding sites on the immobilized anti-human GPLD1 antibody. Excess unbound biotinylated GPLD1 and sample or standard GPLD1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GPLD1 captured in the well and inversely proportional to the amount of GPLD1 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol