What is the catalog number for MTHFD1L ELISA Kit (Bovine)?

The catalog number for MTHFD1L ELISA Kit (Bovine) is OKEH03924-96W.

What sizes are available for MTHFD1L ELISA Kit (Bovine)?

MTHFD1L ELISA Kit (Bovine) is available in the following size(s): 96 Well.

Is MTHFD1L ELISA Kit (Bovine) in stock?

MTHFD1L ELISA Kit (Bovine) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

MTHFD1L ELISA Kit (Bovine)

Product Specifications

CAS Number

7732-18-5

Gene Name

Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like

Gene Aliases

Formyltetrahydrofolate synthetase; monofunctional C1-tetrahydrofolate synthase, mitochondrial.

Gene ID

534296

Accession Number

NP_001069486.1

Reactivity

Bos taurus, Bovine

Target

Monofunctional C1-tetrahydrofolate synthase, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MTHFD1L ELISA Kit (Bovine) (OKEH03924) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MTHFD1L has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MTHFD1L is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MTHFD1L captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Sensitivity

0.089 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

975 kDa

Specificity

Natural and recombinant Bovine Monofunctional C1-tetrahydrofolate synthase, mitochondrial

Protein Length

975

NCBI Gene Symbol

MTHFD1L

Protein Name

Q0VCR7

Gene Name URL

MTHFD1L

Nucleotide Accession Number

NM_001076018.2

Frequently Asked Questions

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