ACACA ELISA Kit (Bovine)
Product Specifications
CAS Number
7732-18-5
Gene Name
Acetyl-CoA carboxylase alpha
Gene Aliases
ACC1; ACCA; ACC-alpha; acetyl-CoA carboxylase 1; acetyl-coenzyme A carboxylase alpha.
Gene ID
281590
Accession Number
NP_776649.1
Reactivity
Bos taurus, Bovine
Target
Acetyl-CoA carboxylase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology ACACA ELISA Kit (Bovine) (OKEH03918) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ACACA antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ACACA and incubated. The ACACA found in the sample or standards competes with the biotinylated ACACA for limited binding sites on the immobilized anti-human ACACA antibody. Excess unbound biotinylated ACACA and sample or standard ACACA is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ACACA captured in the well and inversely proportional to the amount of ACACA which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Sensitivity
29 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
2346 kDa
Specificity
Natural and recombinant Bovine Acetyl-CoA carboxylase 1
Protein Length
2346
NCBI Gene Symbol
ACACA
Protein Name
Q9TTS3
Gene Name URL
ACACA
Nucleotide Accession Number
NM_174224.2
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