CD46 ELISA Kit (Bovine)
Product Specifications
CAS Number
7732-18-5
Gene Name
CD46 molecule
Gene Aliases
Brain microvascular membrane cofactor; CD46 molecule, complement regulatory protein; complement regulatory protein; MCP; membrane cofactor protein; membrane cofactor protein variant 10; membrane cofactor protein variant 7; membrane cofactor protein variant 8; membrane cofactor protein variant 9; soluble membrane cofactor protein variant 11; soluble membrane cofactor protein variant 12; soluble membrane cofactor protein variant 13; trophoblast-lymphocyte cross-reactive antigen.
Gene ID
280851
Accession Number
NP_001229490.1
Reactivity
Bos taurus, Bovine
Target
Membrane cofactor protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CD46 ELISA Kit (Bovine) (OKEH03916) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD46 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD46 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD46 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/ml
Sensitivity
12.3 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
361 kDa
Specificity
Natural and recombinant Bovine Membrane cofactor protein
Protein Length
361
NCBI Gene Symbol
CD46
Protein Name
Q6VE48
Gene Name URL
CD46
Nucleotide Accession Number
NM_001242561.1
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