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CD46 ELISA Kit (Bovine)

Product Specifications

CAS Number

7732-18-5

Gene Name

CD46 molecule

Gene Aliases

Brain microvascular membrane cofactor; CD46 molecule, complement regulatory protein; complement regulatory protein; MCP; membrane cofactor protein; membrane cofactor protein variant 10; membrane cofactor protein variant 7; membrane cofactor protein variant 8; membrane cofactor protein variant 9; soluble membrane cofactor protein variant 11; soluble membrane cofactor protein variant 12; soluble membrane cofactor protein variant 13; trophoblast-lymphocyte cross-reactive antigen.

Gene ID

280851

Accession Number

NP_001229490.1

Reactivity

Bos taurus, Bovine

Target

Membrane cofactor protein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CD46 ELISA Kit (Bovine) (OKEH03916) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD46 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD46 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD46 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

31.2-2000pg/ml

Sensitivity

12.3 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

361 kDa

Specificity

Natural and recombinant Bovine Membrane cofactor protein

Protein Length

361

NCBI Gene Symbol

CD46

Protein Name

Q6VE48

Gene Name URL

CD46

Nucleotide Accession Number

NM_001242561.1

Frequently Asked Questions

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