ECHS1 ELISA Kit (Bovine)
Product Specifications
CAS Number
7732-18-5
Gene Name
Enoyl-CoA hydratase, short chain 1
Gene Aliases
Enoyl CoA hydratase, short chain, 1, mitochondrial; enoyl Coenzyme A hydratase, short chain, 1, mitochondrial; enoyl-CoA hydratase 1; enoyl-CoA hydratase, mitochondrial; SCEH; short-chain enoyl-CoA hydratase.
Gene ID
281748
Accession Number
NP_001020377.2
Reactivity
Bos taurus, Bovine
Target
Enoyl-CoA hydratase, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ECHS1 ELISA Kit (Bovine) (OKEH03903) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ECHS1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ECHS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ECHS1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.78-50ng/mL
Sensitivity
0.33 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
31 kDa
Specificity
Natural and recombinant Bovine Enoyl-CoA hydratase, mitochondrial
Protein Length
290
NCBI Gene Symbol
ECHS1
Protein Name
Q58DM8
Gene Name URL
ECHS1
Nucleotide Accession Number
NM_001025206.2
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