What is the catalog number for NPPC ELISA Kit (Bovine)?

The catalog number for NPPC ELISA Kit (Bovine) is OKEH03865-96W.

What sizes are available for NPPC ELISA Kit (Bovine)?

NPPC ELISA Kit (Bovine) is available in the following size(s): 96 Well.

Is NPPC ELISA Kit (Bovine) in stock?

NPPC ELISA Kit (Bovine) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

NPPC ELISA Kit (Bovine)

Product Specifications

CAS Number

7732-18-5

Gene Name

Natriuretic peptide C

Gene Aliases

C-type natriuretic peptide; natriuretic peptide precursor C; SVSP15.

Gene ID

281356

Accession Number

NP_776550.1

Reactivity

Bos taurus, Bovine

Target

C-type natriuretic peptide

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology NPPC ELISA Kit (Bovine) (OKEH03865) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-bovine NPPC antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated NPPC and incubated. The NPPC found in the sample or standards competes with the biotinylated NPPC for limited binding sites on the immobilized anti-bovine NPPC antibody. Excess unbound biotinylated NPPC and sample or standard NPPC is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated NPPC captured in the well and inversely proportional to the amount of NPPC which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

31.2-2000pg/mL

Sensitivity

13 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

13 kDa

Specificity

Natural and recombinant Bovine C-type natriuretic peptide

Protein Length

126

NCBI Gene Symbol

NPPC

Protein Name

P55206

Gene Name URL

NPPC

Nucleotide Accession Number

NM_174125.2

Frequently Asked Questions

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