LOXL1 ELISA Kit (Bovine)
Product Specifications
CAS Number
7732-18-5
Gene Name
Lysyl oxidase like 1
Gene Aliases
Lysyl oxidase homolog 1; lysyl oxidase-like protein 1.
Gene ID
281903
Accession Number
NP_776808.1
Reactivity
Bos taurus, Bovine
Target
Lysyl oxidase homolog 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LOXL1 ELISA Kit (Bovine) (OKEH03843) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LOXL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LOXL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LOXL1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/mL
Sensitivity
20 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
64 kDa
Specificity
Natural and recombinant Bovine Lysyl oxidase homolog 1
Protein Length
591
NCBI Gene Symbol
LOXL1
Protein Name
Q95L39
Gene Name URL
LOXL1
Nucleotide Accession Number
NM_174383.2
Frequently Asked Questions
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