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INHA ELISA Kit (Bovine)

Product Specifications

CAS Number

7732-18-5

Gene Name

Inhibin subunit alpha

Gene Aliases

Inhibin A; inhibin alpha chain; inhibin alpha subunit; inhibin, alpha.

Gene ID

281254

Accession Number

NP_776519.2

Reactivity

Bos taurus, Bovine

Target

Inhibin alpha chain

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology INHA ELISA Kit (Bovine) (OKEH03841) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for INHA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for INHA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample INHA captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.312-20ng/mL

Sensitivity

0.091 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

39 kDa

Specificity

Natural and recombinant Bovine Inhibin alpha chain

Protein Length

360

NCBI Gene Symbol

INHA

Protein Name

P07994

Gene Name URL

INHA

Nucleotide Accession Number

NM_174094.4

Frequently Asked Questions

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